Application

Research CategorySignaling

Biochem/physiol Actions

Target Sub-Family: Misc

Protein Target: IKKe

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IKBKE is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250µg size as catalog # 14-926M and in bulk as catalog # 14-926-K.

Physical form

Purified by immunoaffinity chromatography.

50mM Tris/HCl pH7.5, 150mM NaCl, 10mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM NaF, 28% v/v glycerol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol

Quality

Routinely evaluated by phosphorylation of casein.

Storage and Stability

6 months at -70°C